Immune Response and Western Blotting

Literature selected by Kathleen M. Dickson
and presented Oct. 9, 1999 for comment at the Lyme-L Discussion List

Infect Immun 1994 Jun 62:6 2625-7

Tick transmission of Borrelia burgdorferi to inbred strains of mice induces an antibody response to P39 but not to outer surface protein A

[published erratum appears in Infect Immun 1995  Mar;63(3):1146]

Golde WT, Kappel KJ, Dequesne G, Feron C, Plainchamp D, Capiau C, Lobet Y

Natural tick transmission of infection by Borrelia burgdorferi induces a very different serum antibody response than needle inoculation of spirochetes. We present data, obtained by using the mouse model, that show that the OspA response was barely detectable, whereas all animals developed significant anti-P39 titers after exposure to B. burgdorferi-infected ticks.

 Infect Immun 1995 Dec 63:12 4795-801

Variation in antigenicity and infectivity of derivatives of Borrelia burgdorferi, strain B31, maintained in the natural, zoonotic cycle compared with maintenance in culture.

Golde WT, Dolan MC

The original isolate of Borrelia burgdorferi, strain B31, can be maintained in vitro indefinitely. A number of studies have demonstrated that there are recognizable changes in the genetic composition of the spirochete after more than 60 passages. We have maintained B31 in the natural zoonotic cycle of transmission of infection between laboratory mice and laboratory-reared Ixodes ticks.

To determine whether similar changes occur in the natural transmission cycle, we reisolated strain B31 from mouse skin at the  5. zoonotic cycle. This reisolated derivative

Analysis of antigen  expression with monoclonal antibodies generated against B31, however, showed differential expression of a subset of antigens between B31 and the isolated derivative.

J Exp Med 1993 Jan 1 177:1 9-17

The major histocompatibility complex-restricted response of recombinant inbred strains of mice  to natural tick transmission of Borrelia burgdorferi.

Golde WT, Burkot TR, Sviat S, Keen MG, Mayer LW, Johnson BJ, Piesman J

The causative agent of Lyme disease, Borrelia burgdorferi, is transmitted by ticks of the Ixodes ricinus complex.

In this study, we report the antibody response of recombinant inbred strains of mice of the H-2b, H-2d, and H-2k haplotypes, infected with B. burgdorferi as a result of exposure to infected I. dammini.

  1. The patterns of antibody response assayed by Western blot analysis indicate significant major histocompatibility complex (MHC) restriction to bacterial antigens within the first 2 mo of infection in mice (i.e. during this period only certain haplotypes have contributed to the immune response, J. Gruber).
  2. Other bacterial antigens induce a significant response across the MHC haplotypes tested
  3. A third group of bacterial antigens appear to generate a MHC-nonrestricted response, and this lack of restriction is maintained when assaying the crossreactivity of the response with other strains of B. burgdorferi. These proteins may provide more accurate diagnostic probes than those currently in use.

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